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Mutagenesis vol. 11 no. 6 pp. 537-546, 1996
© 1996 UK Environmental Mutagen Society/Oxford University Press

research-article

A comparison of conventional metaphase analysis of Giemsa-stained chromosomes with multi-colour fluorescence in situ hybridization analysis to detect chromosome aberrations induced by daunomycin

Sian Ellard1,3, Sheila Toper2, Gill Stemp2, Elizabeth M. Parry1, Phillip Wilcox2 and James M. Parry1

1School of Biological Sciences, University of Wales Swansea Singleton Park, Swansea SA2 8PP 2Glaxo Wellcome Group Research and Development Ltd Park Road, Ware, Herts SGI2 ODP, UK

Chromosome aberrations induced by daunomycin, a widely used positive control compound for in vitro cytogenetics assays, were identified by multi-colour fluorescence in situ hybridization with probes for chromosomes 1, 2 and 3. The frequency and distribution of aberration types were compared to conventional metaphase analysis of Giemsastained chromosomes from parallel human lymphocyte cultures. Multi-colour chromosome painting was a more sensitive method for detecting daunomycin-induced chromosome aberrations compared with conventional metaphase analysis because: (i) a higher level of statistical significance was achieved at low doses; and (ii) the increases in aberration frequencies compared with controls were greater. The majority of exchanges identified by Giemsastaining were unstable and were likely to lead to cell death. In contrast, those detected by FISH were mostly stable exchanges which may be transmitted to cell progeny. Multicolour FISH using whole chromosome probes may provide an elegant solution to the problem of identifying non-lethal, heritable exchange events. The benefit of this technique is the quantification of a cytogenetic endpoint directly associated with carcinogenesis.

3To whom correspondence should be addressed at: Royal Devon and Exeter Hospital NHS Trust, Department of Pathology, Church Lane, Heavitree, Exeter EX2 5AD, UK


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