Skip Navigation

This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Pradheep Kumar, C. P.
Right arrow Articles by Shanmugam, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pradheep Kumar, C. P.
Right arrow Articles by Shanmugam, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Mutagenesis vol. 11 no. 6 pp. 553-557, 1996
© 1996 UK Environmental Mutagen Society/Oxford University Press

research-article

Cytotoxic and genotoxic effects of cleistanthin B in normal and tumour cells

Chhalliyil Prabhakaran Pradheep Kumar, Natarajan Panneerselvam, Sunderasan Rajesh and Govindaswamy Shanmugam1

Department of Biochemistry and Cancer Biology, School of Biological Sciences, Madurai Kamaraj University Madurai 625 021, India

Cleistanthin B, one of the toxic constituents of Cleistanthus collinus, was found to be cytotoxic to normal and tumour cells. In comparison with normal cells, tumour cells were sensitive to lower doses of toxin. The 50{per thousand} growth inhibition GI50 values for normal cell lines were from 2 x 10–5 to 4.7x10–4 M and for tumour cells the values ranged from 1.6x10–6 to 4x10–5 M. Short exposure 30 min of Chinese hamster ovary CHO cells to cleistanthin B at 1–6 µg/ml resulted in extensive chromatid and isochromatid breaks and gaps. However there was no significant increase in cell death and DNA strand breaks in cells treated under the above conditions. Cleistanthin B induced micronucleus formation in cultured lymphocytes in a dose-dependent manner. CHO cells treated with high doses of cleistanthin B showed a decrease in cell viability and a concomitant increase in DNA strand-breaks. The cell death appears to be due to apoptosis since nucleosome-like ladders were observed in the treated cells when the DNA was electrophorized in agarose gels.

1To whom correspondence should be addressed


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.