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Mutagenesis vol. 11 no. 6 pp. 593-596, 1996
© 1996 UK Environmental Mutagen Society/Oxford University Press

research-article

Anti-CD38 prevents the development of the adaptive response induced by X-rays in human lymphocytes

Maria Wojewózka1, Marcin Kruszewski and Irena Szumiel

Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology PL-03-195 Warsaw, Poland

Irradiation of human lymphocytes (1 cGy X-rays, 37°C) evoked an ~30% decrease in the frequency of micronuclei upon subsequent X-irradiation (1.5 Gy). The response was reflected in a lower micronucleus frequency but not in the DNA repair rate measured by the comet assay directly after the challenge dose. Treatment of lymphocytes with anti-CD38 antibody 1 h before irradiation with the adaptive dose prevented the development of the adaptive response measured as micronuclei frequency, but adaptation was not reflected in a lower rate of DNA repair, measured by the alkaline version of the ‘comet’ assay. In lymphocytes that were anti-CD38-treated and irradiated and or irradiated with the adaptive dose the rate of DNA repair was not changed. However, the mean DNA damage level in adapted anti-CD38-treated lymphocytes was significantly lower than that in the control lymphocytes at all time points. We conclude that ligation of CD38 by antibody initiates signalling that prevents the development of the adaptive response induced by X-rays. Lower chromosome damage revealed by the cytokinesis block-micronucleus test in the adapted lymphocytes is unrelated to DNA repair rate.

1To whom correspondence should be addressed


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K. Brzoska and I. Szumiel
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Mutagenesis, January 1, 2009; 24(1): 1 - 8.
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