Mutagenesis, Vol. 14, No. 1, 113-119,
January 1999
© 1999 UK Environmental Mutagen Society/Oxford University Press
A comparison of the effects of diverse mutagens at the lacZ transgene and Dlb-1 locus in vivo
Department of Biology, York University, Toronto, Canada M3J 1P3
Transgenic assays permit the detection of mutations in any tissue, whereas endogenous mutations can be measured in very few. For this reason comparisons between these loci when both can be measured in the same cells are of considerable interest. Previous comparisons have been inconsistent: usually these loci have responded alike, however, in some cases the endogenous locus has been more sensitive and at other times the transgenic locus has been more sensitive. Here we report a comparison of the lacZ transgene of the MutaTMMouse and the endogenous Dlb-1 gene in the epithelium of the small intestine after acute exposure to seven mutagens. Benzo[a]pyrene, 5-bromo-2'-deoxyuridine, methyl methane sulphonate, ethyl methane sulphonate, N-ethyl-N-nitrosourea, mitomycin C and N-methyl-N-nitrosourea were all given by gavage to F1 (MutaMousexSWR) mice. Mutations were quantified 2 weeks after the end of treatment. The data shows that all of the agents induced similar mutant frequencies at the Dlb-1 locus and at the lacZ transgene. The acute treatments generally produced only modest increases in mutant frequency at both loci. The higher background frequency observed at the lacZ transgene reduces the ability of the transgenic assay to detect the same absolute increase in mutant frequency.
1 To whom correspondence should be addressed. Tel: +1 416 736 2100, ext. 33053; Fax: +1 416 736 5698; Email: lidiac{at}yorku.ca and jheddle@yorku.ca
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  E. Bilsland and J. A. Downs Tails of histones in DNA double-strand break repair Mutagenesis, May 1, 2005; 20(3): 153 - 163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-L. Song, G. J.S. Jenkins, J. Ashby, H. Tinwell, and J. M. Parry The application of the restriction site mutation assay to compare 1-ethyl-1-nitrosourea-induced mutations between the endogenous p53 gene and the transgenic LacZ gene in MutaMouse testes Mutagenesis, January 1, 2001; 16(1): 59 - 64. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. R. Clarke Manipulating the germline: its impact on the study of carcinogenesis Carcinogenesis, March 1, 2000; 21(3): 435 - 441. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Heddle On clonal expansion and its effects on mutant frequencies, mutation spectra and statistics for somatic mutations in vivo Mutagenesis, May 1, 1999; 14(3): 257 - 260. [Full Text] [PDF]
|
|