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Mutagenesis, Vol. 14, No. 4, 437-438, July 1999
© 1999 UK Environmental Mutagen Society/Oxford University Press

Short Communication

Modification of the Comet assay for the detection of DNA strand breaks in extremely small tissue samples

Robert S. Tebbs, James E. Cleaver, Roger A. Pedersen1 and Andreas Hartmann2,3

Department of Dermatology, University of California, 1 Reproductive Genetics Unit, Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, CA 94143, USA and 2 Novartis Pharma AG, Genetic Toxicology, WSH 2881.5.14, CH-4002 Basel, Switzerland

We modified the Comet assay to enable the quantification of DNA strand breakage in individual cells of extremely small tissue samples. This modification was used to analyze cells isolated from the ectoplacental cone and egg cylinder of mouse embryos at embryonic day 7.5. We detected more naturally occurring DNA strand breaks and a higher number of apoptotic cells in the ectoplacental cone compared with the egg cylinder.

3 To whom correspondence should be addressed. Tel: +41 61 324 1951; Fax: +41 61 324 1274: Email: andreas.hartmann{at}pharma.novartis.com


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