Mutagenesis, Vol. 14, No. 6, 633-638,
November 1999
© 1999 UK Environmental Mutagen Society/Oxford University Press
Analysis of inversions and sister chromatid exchanges in chromosome 3 of human lymphocytes exposed to X-rays
1 Institute of Genetics, University of Essen, FB-9, D-45171 Essen, Germany, 2 Institute of Chemistry and Nuclear Technology, Department of Radiobiology and Health Protection, PO Box 97, ul. Dorodna 16, 00-981 Warszawa, Poland and 3 Inner Clinics and Policlinics, University Clinics Essen, D-45122 Essen, Germany
It has been shown repeatedly that exposure of G1 cells unifilarily labelled with 5-bromodeoxyuridine (BrdU) to X-rays leads to sister chromatid exchanges (SCE) when the cells are allowed to grow for one further cycle in the absence of BrdU. It has been suggested that damage induced by ionizing radiation does not lead to `true' SCE and that the observed SCE are `false', resulting from structural chromosomal aberrations, especially interstitial inversions. We used a painting probe for the p14 region of human chromosome 3 and anti-BrdU antibodies to analyse the frequency of radiation-induced SCE in that chromosome. This method allowed us to discriminate between para- and pericentric `true' and `false' SCE. Our results indicate that most radiation-induced SCE do not result from inversions.
* To whom correspondence should be addressed. Tel: +49 201 183 3388; Fax: +49 201 183 2866; Email: guenter.obe{at}uni-essen.de
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