Mutagenesis, Vol. 16, No. 1, 17-23,
January 2001
© 2001 UK Environmental Mutagen Society/Oxford University Press
Heritable effects of paternal irradiation in mice on signaling protein kinase activities in F3 offspring
Institute of Toxicology and Environmental Health, Old Davis Road, University of California, Davis, CA 95616, USA
We evaluated F3 mouse offspring from paternal F0 attenuated 137Cs 
-irradiation (1.0 Gy) for heritable effects on gene products that can modulate cell proliferation rate and that may be markers for genomic instability. The F3 generation was selected for evaluation as a stringent test for heritability of effects from paternal F0 germline irradiation. Male CD1 mice were bred 6 weeks after irradiation so that the fertilizing sperm were type B spermatogonia at the time of irradiation. The resulting F1 males were bred to CD1 females to produce F2 four-cell embryos. The F2 embryos with a radiation history were paired with `control' CD1 four-cell embryos that were heterozygous for the neo transgene. These F2 XY–XY chimeras, consisting of cells derived from both an embryo with a paternal F0 radiation history and a control embryo, were transferred to foster mothers, raised to adulthood and bred to produce F3 offspring. F3 offspring were evaluated for hepatic activities of receptor tyrosine kinase, protein kinase C and MAP kinase and for protein levels of nuclear p53 and p21waf1. All three protein kinase activities were altered and nuclear levels of p53 and p21waf1 protein were higher in the group of offspring that included F3 offspring with a paternal F0 radiation history than in littermates in the neo-positive control group. To our knowledge, this is the first observation in the descendants of paternal germline irradiation of effects on signal protein kinase activities and downstream nuclear target proteins that can influence cell proliferation rates.
1 To whom correspondence should be addressed. E-mail: jebaulch{at}ucdavis.edu
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  J. E. Baulch and O. G. Raabe Gamma irradiation of Type B spermatogonia leads to heritable genomic instability in four generations of mice Mutagenesis, September 1, 2005; 20(5): 337 - 343. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Barber, M. A. Plumb, E. Boulton, I. Roux, and Y. E. Dubrova Elevated mutation rates in the germ line of first- and second-generation offspring of irradiated male mice PNAS, May 14, 2002; 99(10): 6877 - 6882. [Abstract] [Full Text] [PDF]
|
|