Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (4)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Morales, J. F.
Right arrow Articles by Murnane, J. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Morales, J. F.
Right arrow Articles by Murnane, J. P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Mutagenesis, Vol. 18, No. 2, 151-158, March 2003
© 2003 UK Environmental Mutagen Society/Oxford University Press

Environmental factors affecting transcription of the human L1 retrotransposon. II. Stressors

José F. Morales, Elizabeth T. Snow1 and John P. Murnane2

Radiation Oncology Research Laboratory, University of California–San Francisco, 1855 Folsom Street, MCB 200, San Francisco, CA 94103, USA and 1 Centre for Cellular and Molecular Biology, School of Biological and Chemical Sciences, Deakin University, 221 Burwood Highway, Burwood, 3125 Victoria, Australia

Retrotransposons have clearly molded the structure of the human genome. The reverse transcriptase coded for by long interspersed nuclear elements (LINEs) accounts for 35% of the human genome, with 8–9 x 105 copies of the most common human LINE element, L1Hs. Retrotransposons cycle through an RNA intermediate with transcription as the rate limiting step. Because various retrotransposons have been demonstrated to be induced by environmental stimuli, we investigated the response of the L1Hs promoter to various agents. L1Hs promoter activity was analyzed by transfecting an L1Hs-expressing cell line with plasmids containing one of two L1Hs promoters fused to the LacZ reporter gene. L1Hs promoter activity was then monitored with a ß-galactosidase assay. Treatment with UV light and heat shock resulted in a small increase in ß-galactosidase activity from one promoter, while treatment with tetradecanoylphorbol 13-acetate resulted in small increases in ß-galactosidase activity from both promoters. No increase in ß-galactosidase activity was observed after exposure to X-rays or hydrogen peroxide.

2 To whom correspondence should be addressed: Tel: +1 415 476 9083; Fax: +1 415 476 9069; Email: murnane{at}rorl.ucsf.edu


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol Biol EvolHome page
S. Iwashita, S. Ueno, K. Nakashima, S.-Y. Song, K. Ohshima, K. Tanaka, H. Endo, J. Kimura, M. Kurohmaru, K. Fukuta, et al.
A Tandem Gene Duplication Followed by Recruitment of a Retrotransposon Created the Paralogous Bucentaur Gene (bcntp97) in the Ancestral Ruminant
Mol. Biol. Evol., April 1, 2006; 23(4): 798 - 806.
[Abstract] [Full Text] [PDF]

Home page
 Journal of the American Dental AssociationHome page
F. RAMOS-GOMEZ, G. D. CRUZ, M. R. WATSON, M. T. CANTO, and A. E. BONETA
Latino oral health: A research agenda toward eliminating oral health disparities
J Am Dent Assoc, September 1, 2005; 136(9): 1231 - 1240.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.