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Mutagenesis, Vol. 18, No. 3, 273-275, May 2003
© 2003 UK Environmental Mutagen Society/Oxford University Press

The effect of aging on the results of the rat micronucleus assay

Shuichi Hamada3, Kazuo Nakajima1, Tadao Serikawa1 and Makoto Hayashi2

Central Research Laboratory, SSP Co. Ltd, 1143 Nanpeidai, Narita, Chiba 286-8511, Japan, 1 Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan and 2 National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan

We conducted the micronucleus assay in 3-, 5-, 7-, 9-, 11- and 13-week-old male rats to determine whether the results varied with age. We administered cyclophosphamide orally at 0 (vehicle), 5, 10 or 20 mg/kg, twice, 24 h apart, to five rats per dosage group and collected bone marrow and peripheral blood 24 h after the second treatment. We observed an age-related decrease in micronucleus-inducing activity in both polychromatic erythrocytes (bone marrow) and reticulocytes (peripheral blood), which we attributed to an age-related decrease in hematopoiesis. In spite of the age-related decrease in sensitivity to the model chemical studied here, all age groups tested showed positive responses. We concluded that the rat is suitable for the micronucleus assay regardless of age.

3 To whom correspondence should be addressed. Tel: +81 476 27 1511; Fax: +81 476 26 7948; Email: shuichi.hamada{at}ssp.co.jp


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