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Mutagenesis vol. 18 no. 6 pp. 545-548, November 2003
© 2003 UK Environmental Mutagen Society/Oxford University Press

Genotoxic and protective effects of hyperbaric oxygen in A549 lung cells

Günter Speit1 and Irina Bonzheim

Universitätsklinikum Ulm, Abteilung Humangenetik, D-89070 Ulm, Germany

The human lung cell line A549 is frequently used as an in vitro model for studying lung toxicity and genotoxicity of environmental mutagens and carcinogens. Hyperbaric oxygen (HBO) treatment has been shown to be an excellent model for investigating the genetic consequences of oxidative stress in vitro and in vivo. We have now studied the genotoxic effects of and adaptive protection by HBO in A549 cells. Using the alkaline Comet assay, our results show that HBO exposure directly induces DNA damage but reduces the DNA-damaging effect of a second HBO treatment of the cells 24 h later. HBO pretreatment also reduces potassium chromate-induced genotoxicity in A549 cells. Heme oxygenase-1 (HO-1) protein level is increased 24 h after HBO exposure and inhibition of HO-1 activity by tin-mesoporphyrin increased HBO genotoxicity. These results are in accordance with previous findings suggesting an involvement of HO-1 in the adaptive protection. Our study indicates that A549 cells are an appropriate cell culture system for the evaluation of genetic effects induced by HBO in lung cells.

1To whom correspondence should be addressed. Tel: +49 731 500 23429; Fax: +49 731 500 23438; Email: guenter.speit{at}medizin.uni-ulm.de


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