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Mutagenesis vol. 2 no. 5 pp. 371-374, 1987
© 1987 UK Environmental Mutagen Society/Oxford University Press

other

The effect of 9-ß-D-arabinofuranosyladenine on the formation of X-ray induced chromatid aberrations in X-irradiated G2 human cells

Hossein Mozdarani and Peter E. Bryant1

Department of Anatomy and Experimental Pathology, University of St Andrews, St Andrews Fife KY16 9TS, UK

The effects of the nucleoside analogue 9-ß-D-arabinofurano-syladenine (ara A) alone or in combination with X-rays on the induction of G2-phase chromosomal damage were studied in immortalized human fibroblasts of lung origin (MRC5-SV1). Ara A is known to be an S-phase specific clastogen, a powerful inhibitor of DNA synthesis and an inhibitor of DNA double-strand break (dsb) repair. The length of the G2-phase of this cell line could be defined as {small tilde} 4 h from data for treatment of cells with ara A alone in which a sharp rise in number of chromatid aberrations was found to occur when ara A treatment times exceeded 4 h; i.e. when cells were in late S-phase at the time of treatment. The frequency of chromatid deletions in X-irradiated G2-phase cells was found to decrease as the time between irradiation and fixation increased. This was interpreted as reflecting the underlying repair of dsb. When X-irradiated cells were treated with ara A between irradiation and fixation, the decrease in deletions found after X-rays alone was not observed. This was interpreted as reflecting the inhibition of dsb repair. Unlike deletions, the yield of exchanges increased during incubation of G2-phase cells after X-ray exposure and the rate of this increase was found to be trebled by the addition of ara A to the medium. We postulate that the increased rate of exchange aberration formation in the absence of dsb repair indicates the existence of a second ‘error-prone’ misjoining mechanism which is independent of DNA synthesis.

1To whom correspondence should be addressed


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P. E. Bryant and H. Mozdarani
A comparison of G2 phase radiation-induced chromatid break kinetics using calyculin-PCC with those obtained using colcemid block
Mutagenesis, September 1, 2007; 22(5): 359 - 362.
[Abstract] [Full Text] [PDF]


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