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Mutagenesis 2005 20(2):147-150; doi:10.1093/mutage/gei021
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© The Author 2005. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. All rights reserved. For permissions, please email: journals.permissions@oupjournals.org

The genotoxic effects of hepatitis B virus to host DNA

Pinar Özkal1,*, Hatice Ilgin-Ruhi2, Meral Akdogan4, Atilla Halil Elhan3, Sabite Kaçar4 and Nurgül Sasmaz4

1Department of Medical Biology, 2Department of Medical Genetics and 3Department of Biostatistics, Ankara University School of Medicine, Ankara, Turkey and 4Department of Gastroenterology, Türkiye Yüksek Yhtisas Hospital, Ankara, Turkey

Chronic viral hepatitis is the main cause of chronic liver disease, cirrhosis and hepatocellular carcinoma throughout the world. Hepatitis B virus (HBV) has mutagenic effects on somatic cells. HBV may be showing these mutagenic effects through its viral proteins or through integrating into host DNA. The aim of this study was to determine whether HBV has a genotoxic effect on host DNA or not. Peripheral blood lymphocytes of 31 chronic HBV patients and 20 chronic HBV carriers were cultured in order to make cytogenetic evaluation by observing chromosome breakage and cytological evaluation by the micronucleus (MN) test. Their results were compared with 20 healthy controls. For each individual, 100 metaphase chromosome spreads were analysed. Around 190–1091 binucleated cells were observed and MN were scored for each individual. Our results showed significantly higher frequencies of chromosome breaks in chronic HBV patients and in HBV carriers than in the control group. There was no difference in MN scores among HBV patients, HBV carriers and healthy carriers. Based on our data, we conclude that chronic HBV patients and carriers have chromosomal instability and that HBV carriers are as affected as patients because of their same chromosome breakage levels.

* To whom correspondence should be addressed at: Ankara Üniversitesi Tip Fakültesi, Tibbi Biyoloji Anabilim Dali, Morfoloji Binasi 06100 Sihhiye, Ankara, Türkye. Tel: +90 312 310 30 10/401; Fax: +90 312 310 6370; Email: ozkal{at}medicine.ankara.edu.tr


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