Skip Navigation


Mutagenesis Advance Access originally published online on March 6, 2008
Mutagenesis 2008 23(4):267-270; doi:10.1093/mutage/gen009
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
23/4/267    most recent
gen009v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Shovman, O.
Right arrow Articles by Bryant, P. E.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shovman, O.
Right arrow Articles by Bryant, P. E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?


© The Author 2008. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org.

An improved assay for radiation-induced chromatid breaks using a colcemid block and calyculin-induced PCC combination

Olga Shovman, Andrew C. Riches, Douglas Adamson1 and Peter E. Bryant*

Bute Medical School, University of St Andrews, St Andrews, KY19 9TS, UK 1Ward 32, Ninewells Teaching Hospital, University of Dundee, Dundee DD1 9SY, UK

We report on a new method for the study of radiation-induced chromatid breaks in stimulated human peripheral blood T lymphocytes, involving a combination of a 1-h colcemid block and a short (15 min) calyculin A treatment. We find that this procedure eliminates the problem of centromere splitting when calyculin A is used alone for a longer period and produces metaphase spreads with superior quality. By this procedure, the chromosomes and the chromatid breaks are expanded and thereby make for improved break scoring. In a comparison of the new technique with the conventional colcemid block method, we show a close proportionality between the frequencies of chromatid breaks scored with the two methods. The frequency of chromatid breaks with the new method was found to be significantly higher than that with colcemid alone, adding a higher sensitivity to the assay as an additional advantage.

* To whom correspondence should be addressed. Tel: +44 (0)1334 463510; Fax: +44 (0)1334 463482; Email: peb{at}st-and.ac.uk

Received on December 5, 2007; revised on January 17, 2008; accepted on January 25, 2008.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.