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Mutagenesis vol. 4 no. 3 pp. 174-178, 1989
© 1989 UK Environmental Mutagen Society/Oxford University Press

research-article

Induction of kinetochore positive and negative micronuclei in V79 cells by the alkylating agent diethylsulphate

Michael Nüsse, Silvia Viaggi1 and Stefania Bonatti1

Gesellschaft für Strahien-und Umweltforschung, Institut für Biophysikalische Strahlenforschung Paul-Ehrlich-Strasse 20, 6000 Frankfurt/M-70, FRG 1Istituto Nazionale per la Ricerca sul Cancro, Laboratory of Mutagenesis Viale Benedeno XV No. 10, 16132 Genova, Italy

The induction by diethylsulphate of micronuclei derived from acentric fragments or from whole chromosomes was studied in Chinese hamster V79 cells using autoantibodies from the serum of a scieroderma patient (CREST-syndrome) to detect centromere-kinetochore structures. Centromere-contaimng micronuclei appeared early after treatment and plateaued both earlier and at lower level than centromere-lacking micro-nuclei. The frequency of centromere-containing micronuclei was in good agreement with that of mitotic chromosome displacement, suggesting that a high proportion of displaced chromosomes were transmitted to the cytoplasm of one of the two daughter cells, where they gave rise to micronuclei. On the contrary, centromere-lacking micronuclei were more frequent than what could be expected from chromosome fragments observed in mitotic stages.


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