Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Norimura, T.
Right arrow Articles by McCormick, J.J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Norimura, T.
Right arrow Articles by McCormick, J.J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Mutagenesis vol. 5 no. 5 pp. 447-451, 1990
© 1990 UK Environmental Mutagen Society/Oxford University Press

research-article

Cytotoxic and mutagenic effect of UV, ethylnitrosourea and (±)-7ß8,8{alpha}-dihydroxy-9{alpha},10{alpha}-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene in diploid human T lymphocytes in culture: comparison with fibroblasts

T. Norimura1, V.M. Maher2 and J.J. McCormick

Carcinogenesis Laboratory, Fee Hall, Department of Microbiology and Department of Biochemistry, Michigan State University East Lansing, MI 48824-1316, USA 1Present address: Department of Radiation Biology and Health, University of Occupational and Environmental Health (Japan) 1-1 Iseigaoka Yahatanishi-ku, Kitakyushu 807, Japan

The cytotoxic and mutagenic effects of a series of carcinogenic mutagens in human peripheral blood T cells exposed in culture were determined and compared with results obtained previously with diploid human skin fibroblasts. The mutagens studied were: ethylnitrosourea (ENU), (±)-7ß3,8{alpha}-dihydroxy-9{alpha},10{alpha}-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE) and UV (254 nm) radiation. Resistance to 6-thioguanine (TG) served as the genetic marker. The T lymphocytes, cultured in the presence of 10% fetal bovine serum, T cell growth factor and lethally-irradiated B lymphoblastoid cells (allogeneic stimulators), exhibited population doubling times of 22 h for as long as tested, i.e. >40 days, and cloning efficiencies between 30 and 50%. The T cells were exposed to the mutagens in exponential growth, 3 days after being isolated and primed to begin blast formation, using doses that reduced cell survival to between 80 and 25% of the untreated control. The background frequency of TG-resistant cells in the 14 independent populations assayed ranged from 1.2x10–6 to 10.6x10–6, with an average of 5.1x10–6 ± 2.8 x10–6. Each agent induced a dose-dependent increase in TG-resistant cells, reaching 85x10–6 for UV, 260x10–6 for ENU and 70x10–6 for BPDE. When compared on the basis of fluence or applied concentration, T cells were 1.5-times more sensitive than fibroblasts to the cytotoxic effects of ENU, but equally as sensitive as fibroblasts to its mutagenic effects. They were 2.7-times more sensitive than fibroblasts to BPDE-induced cytotoxicity, but 2-times less sensitive to its mutagenicity. T lymphocytes and fibroblasts were about equal in sensitivity to the cytotoxic effect of UV, but T cells were 2-times less sensitive to the mutagenic effects of UV.

2To whom correspondence should be addressed


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.