Mutagenesis vol. 8 no. 3 pp. 207-220, 1993
© 1993 UK Environmental Mutagen Society/Oxford University Press
research-article |
Genome wide spontaneous mutation in human cells determined by the spectrum of mutations in hprt cDNA genes
Department of Molecular Genetics, Gorlaeus Laboratoria University of Leiden PO Box 9502, 2300 RA Leiden 1MGC-Department of Radiation Genetics and Chemical Mutagenesis University of Leiden Wassenaarseweg 72, 2300 RA Leiden, The Netherlands
We have studied spontaneous mutagenesis in five hprt cDNA genes integrated at five different genomic positions in a human lymphoblastoid cell line (TK6). The spectra of 40 mutants from each position were combined to obtain a mutation spectrum of the overall genome. This collection of mutants was used to assess the contribution of several mutagenic processes to spontaneous mutagenesis. Deletions and single base pair changes account for the majority of the mutants and arise in approximately equal amounts (43 and 41%,respectively). The majority of the deletions and insertions are >5 bp and are likely to be caused by template-directed misalignment (slippage) during replication. To account for frameshifts at non-iterated sites we propose a slightly different template-directed replication error model. A considerable amount of the observed base pair changes can also be explained by this last model but several other processes leading to base pair changes such as depurination, deamination or spontaneously arising DNA damage are likely to contribute as well. We have compared this spectrum with mutation spectra in the endogenous hprt genes using published mutation data. It is shown that in the endogenous genes the contribution of base pair substitutions is much larger (71%) than in the hprt cDNA integrates and that deletions are less frequently observed (20%) The mutation rates of the integrated hprt cDNA genes show a mean increase of 30-foldas compared with the endogenous hprt gene. This results in a 60-fold increase of the absolute rate of deletion in the hprt cDNA genes and in a 15-fold increase of the base pair substitution rate. Replication errors such as slippage or the mechanism proposed in this study probably account to a large extent for this increase.
2To whom correspondence should be addressed
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. J. Lippert, J. A. Freedman, M. A. Barber, and S. Jinks-Robertson Identification of a Distinctive Mutation Spectrum Associated with High Levels of Transcription in Yeast Mol. Cell. Biol., June 1, 2004; 24(11): 4801 - 4809. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. E. Hudson, U. Bergthorsson, and H. Ochman Transcription increases multiple spontaneous point mutations in Salmonella enterica Nucleic Acids Res., August 1, 2003; 31(15): 4517 - 4522. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Zhuang, C. Lin, D. Lin, and G. N. Wogan Mutagenesis associated with nitric oxide production in macrophages PNAS, July 7, 1998; 95(14): 8286 - 8291. [Abstract] [Full Text] [PDF]
|
|