Skip Navigation


Mutagenesis Advance Access originally published online on May 21, 2008
Mutagenesis 2008 23(5):367-370; doi:10.1093/mutage/gen028
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
23/5/367    most recent
gen028v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Ahuja, A. K.
Right arrow Articles by Dubrova, Y. E.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ahuja, A. K.
Right arrow Articles by Dubrova, Y. E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?


© The Author 2008. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org.

The effects of Atm haploinsufficiency on mutation rate in the mouse germ line and somatic tissue

Akshay K. Ahuja, Ruth C. Barber, Robert J. Hardwick, Michael M. Weil1, Paula C. Genik1, David J. Brenner2 and Yuri E. Dubrova*

Department of Genetics, University of Leicester, Leicester LE1 7RH, UK 1Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO 80523, USA 2Center for Radiological Research, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA

Using single-molecule polymerase chain reaction, the frequency of spontaneous and radiation-induced mutation at an expanded simple tandem repeat (ESTR) locus was studied in DNA samples extracted from sperm and bone marrow of Atm knockout (Atm+/–) heterozygous male mice. The frequency of spontaneous mutation in sperm and bone marrow in Atm+/– males did not significantly differ from that in wild-type BALB/c mice. Acute exposure to 1 Gy of {gamma}-rays did not affect ESTR mutation frequency in bone marrow and resulted in similar increases in sperm samples taken from Atm+/– and BALB/c males. Taken together, these results suggest that the Atm haploinsufficiency analysed in our study does not affect spontaneous and radiation-induced ESTR mutation frequency in mice.

* To whom correspondence should be addressed. Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, UK. Tel: +44 116 252 5654; Fax: +44 116 252 3378; Email: yed2{at}le.ac.uk

Received on March 14, 2008; revised on April 23, 2008; accepted on April 24, 2008.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.