Mutagenesis, Vol. 14, No. 3, 257-260,
May 1999
© 1999 UK Environmental Mutagen Society/Oxford University Press
Discussion Forum |
On clonal expansion and its effects on mutant frequencies, mutation spectra and statistics for somatic mutations in vivo
Department of Biology, York University, Toronto, Ontario M3J 1P3, Canada
Jackpots of mutations are the bane of transgenic mutation assays, for they can produce large mutant frequencies even in the absence of a mutagen. A jackpot is a large number of mutants that arose from a single mutation. They arise when there is a clonal expansion of a mutated cell. Jackpots as large as 1000 mutants/100 000 plaques have been observed, a mutant frequency of 1%. Such a jackpot can swamp not only the spontaneous mutant frequency but even the mutant frequency induced by potent mutagens, which are rarely >0.2% with even the most potent mutagens and are usually much lower (Hoorn et al., 1993
; Tao et al., 1993; Morrison and Ashby, 1994; Douglas et al., 1995; Mirsalis et al., 1995; Skopek et al., 1995; Cosentino and Heddle, 1999). Very large jackpots are not normally a problem, for
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