Mutagenesis, Vol. 14, No. 6, 639-656,
November 1999
© 1999 UK Environmental Mutagen Society/Oxford University Press
Meeting Report |
Abstracts of the United Kingdom Environmental Mutagen Society 23rd Annual General Meeting, June 23–25, University of Ulster, Coleraine, Northern Ireland, UK
1. Novel role for DNA repair in the genotoxic effects of haloalkanesNieves Abril and Geoff Margison
CRC Section of Genome Damage and Repair, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester M20 4BX, UK
Halogenated aliphatic hydrocarbons such as 1,2-dibromoethane (DBE) and dibromomethane (DBM) have been widely used as pesticides, solvents and intermediates in agricultural and industrial chemistry. They are mutagens and are carcinogenic, teratogenic and embryotoxic in laboratory animals. The two principal routes of metabolic transformation of DBA in mammalian cells are GST-mediated conjugation with GSH and cyP450-mediated oxidation.
The effect of expression of the DNA repair protein, O6-alkylguanine-DNA alkyltransferase, on the genotoxic effects of DBM and DBE was determined in Chinese hamster lung fibroblasts transfected with and expressing high levels of Escherichia coli alkyltransferase (ATase) genes. These included the ogt gene and complete or truncated versions of the E.coli ada gene encoding either O6-alkylguanine (O6-alkG) or alkylphosphotriester (alkPT) ATase activities. As previously observed,
2. DNA repair in plants studied by the comet assay
3. DNA damage and apoptosis
4. Two forms of coal tar genotoxicity and mutagenicity evaluation and analysis of their phototoxicity and photogenotoxicity
5. Lack of genotoxic activity of trichloroethylene and its metabolite S-(1,2-dichlorovinyl)-L-cysteine in rat renal proximal tubular cells in vivo using the comet assay
6. Evaluation of the direct count scoring method for the autoradiographic unscheduled DNA synthesis assay
Reference
7. PRINS localisation of the telomeric sequence (TTAGGG)n in the rat karyotype indicates the presence of telomeric-like sites at the centromeres of some chromosomes
References
8. Alkali sensitive DNA strand breaks and evidence of repair in primary cells and tissue comparable cell lines after UV and clastogen exposure
9. DNA damage, toxic vents and environmental contamination
10. Uracil misincorporation in human DNA detected using a novel variation of the comet assay
References
11. The polymerase arrest assay
12. The mouse lymphoma assay (MLA) using the microtitre methodology: historical data and cell cleansing
13. Mutagenicity of 2-amino-1-methyl-6-phenylimidazo [4,5-b] pyridine (PhIP) in the germ cells of male lacZ transgenic mice (MutaTMMouse)
14. Inducible global genomic repair but constitutive transcription-coupled DNA repair in human cells
References
15. The Mammalian Gene Mutation Database
16. Preliminary investigation into blood biomarkers of human exposures to N-nitroso compounds
17. Toxicological evaluation of a herbal product
References
18. The development of the polymerase inhibition assay for the sensitive detection of DNA damage induced by test chemical agents
19. The effect of acetonitrile on the incidence of micronucleated polychromatic erythrocytes in mouse bone marrow and peripheral blood
20. Interaction between DNA mismatch repair and damaged DNA in human cells
21. The single-laser flow cytometric micronucleus test: a timecourse study using colchicine and urethane in rat and mouse peripheral blood
References
22. The interpretation of the biological relevance of genotoxicity test results: the importance of thresholds
23. Mutations in the XPD DNA repair/transcription gene: site of mutation determines cellular defect and clinical outcome
References
24. The comet assay and male infertility
25. The effect of chlorophyllin on the formation of PhIP-DNA adducts in humans
26. The polymerase stop assay for the determination of DNA adduct frequency on the human p53 gene exposed to increasing doses of UVB and UVC radiation
References
27. Exfoliated breast milk cells in the comet assay: a novel and non-invasive means of assessing genotoxicity in mammary epithelial cells
References
28. The comet assay as a pre-screen for potential L5178Y positives
29. High voltage electric field induced DNA damage, nuclear lamin disassembly and cell cycle dependent apoptosis
30. The immunological consequences of UVB-induced DNA damage
31. Are aneugens mutagenic in the mouse lymphoma TK assay?
References
32. The use of flow cytometry as a predictor of cytotoxicity
33. Detection of oxidative DNA damage with the comet assay: utilisation as a biomarker for potential cancer prevention by nutrients
34. Comparison of acute and sub-acute dosing of 2-acetylaminofluorene (AAF) in the rat bone marrow and peripheral blood micronucleus test
35. Preferential rejoining of 
-radiation induced DNA strand breaks in the p53 domain of J82 bladder carcinoma cells
Reference
36. The endogenous hormone 17ß-estradiol at low concentrations induces DNA damage in MCF-7 breast cancer cells
37. Mutagenesis and repair of 8-oxoguanine in human cells
38. PhIP mutagenicity in the kidney of male MutaTMMouse mice
References
39. Comparative study of the p53 gene and LacZ gene mutations in MutaTMMouse testes treated with ENU
References
40. Genetic effects of oxidative stress
41. N-acetyltransferases (NAT) and sulphotransferases activate heterocyclic amines in the human breast, but NAT mRNA levels are not influenced by NAT genotype
42. An investigation into the effects of colchicine on chromosome loss and non-disjunction in cell lines with varying p53 status
43. Antioxidant/pro-oxidant effects of dietary components: in vitro assessment using the comet assay
44. An investigation of the effects of MDR-1 expression in the haemopoietic compartment on etoposide-induced genotoxicity
45. The BrdU–comet assay: a novel assay for the detection of replication in individual cells
46. Benign prostatic tissues express CYP1 and NAT genes, and activate potential prostate carcinogens
47. The use of primary human cell types for aneugen screening in the in vitro cytokinesis blocked micronucleus assay
Reference
48. Fluorescence in situ hybridisation applied to the embryos of the marine polychaete: Pomatoceros lamarckii
49. The heterocyclic amine PhIP causes S phase cell cycle delay, apoptosis and hprt gene mutations in human lymphoblastoid TK6 cells