Skip Navigation

This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (4)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Espinosa-Aguirre, J.J.
Right arrow Articles by Asteinza, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Espinosa-Aguirre, J.J.
Right arrow Articles by Asteinza, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Mutagenesis vol. 12 no. 3 pp. 159-162, 1997
© 1997 UK Environmental Mutagen Society/Oxford University Press

research-article

Characterization of the CPY isozyme profile induced by cyclohexanol

J.J. Espinosa-Aguirre1,2,3, J. Rubio1, I. LÔpez2, R. Nosti2 and J. Asteinza1

1Institute de Investigaciones Biomédicas, Universidad National Autónoma de México Apartado Postal 70228, 04510 México, D.F. 2Unidad de Investigation en Salud Infantil, Instituto Nacional de Pediatria Apartado Postal 101-54(54), Av. Insurgentes Sur, 3700-C, 04530 Mexico, D.F., Mexico

In a previous report we described the ability of cyclohexanol to induce CYP activity. In order to characterize this induction we tested the capacity of liver S9 from rats orally treated with cyclohexanol for 5 days, to activate several carcinogenic nitrosamines into mutagens in the Salmonella typhimurium TA100 test system. Additionally, Western blot analysis of hepatic microsomes from the same treated animals were analysed with specific antibodies against P450 protein families 1A1/A2, 2B1/B2 and 2E1. Cyclohexanol-S9 mixture was more efficient in activating the following nitrosamines: N-nitrosodimethylamine (NDMA), N-nitrosodipropylamine (NDPA), N-nitrosomethylpropylamine (NMPA), N-nitrosodibutylamine (NDBA), and N-nitrosopyrrolidine (NPYR) into bacterial mutagens than S9 from non-treated animals. The mutagenicity of N-nitrosodiethylamine (NDEA) was not modified in the presence of S9 from cyclohexanol-treated animals. Since the main metabolic pathway leading to the production of mutagenic intermediates of NDMA and NPYR is catalysed by isozyme CYP2E1 and that of NDPA, NMPA and NDBA by CYP2B1/B2, mutagenicity experiments predicted that cyclohexanol induces these two P450 isozyme families. Western blot analysis confirmed the results of the mutagenicity assay, showing an increase in the intensity of CYP2E1 and CYP2B1/B2 protein bands in hepatic microsomes from cyclohexanol treated rats in comparison with non-treated controls. Bacterial mutagenicity tests with specific pro-mutagens were good predictors of the P450 induction properties of cyclohexanol.

3To whom correspondence should be addressed at: Instituto de Investigaciones Biomédicas, Universidad Nacional Autonoma de Mexico, Apartado Postal 70228, 04510 Mexico, D.F., México


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Toxicol SciHome page
O. Beltran-Ramirez, L. Aleman-Lazarini, M. Salcido-Neyoy, S. Hernandez-Garcia, S. Fattel-Fazenda, E. Arce-Popoca, J. Arellanes-Robledo, R. Garcia-Roman, P. Vazquez-Vazquez, A. Sierra-Santoyo, et al.
Evidence that the Anticarcinogenic Effect of Caffeic Acid Phenethyl Ester in the Resistant Hepatocyte Model Involves Modifications of Cytochrome P450
Toxicol. Sci., July 1, 2008; 104(1): 100 - 106.
[Abstract] [Full Text] [PDF]

Home page
 MutagenesisHome page
D. Escobar-Garcia, R. Camacho-Carranza, I. Perez, V. Dorado, M. Arriaga-Alba, and J.J. Espinosa-Aguirre
S9 induction by the combined treatment with cyclohexanol and albendazole
Mutagenesis, November 1, 2001; 16(6): 523 - 528.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.