Mutagenesis, Vol. 14, No. 1, 57-61,
January 1999
© 1999 UK Environmental Mutagen Society/Oxford University Press
Dual-colour FISH analysis to characterize a marker chromosome in cytochrome P450 2B1 recombinant V79 Chinese hamster cells
1 Genetica e Biochimica Tossicologica, Istituto di Mutagenesi e Differenziamento, CNR, via Svezia 10, 56124 Pisa, Italy, 2 MGC, Department of Radiation Genetics and Chemical Mutagenesis, Leiden University, Medical Centre, Wassenaarseweg 72, 2333 AL, Leiden, The Netherlands and 3 Institut fur Toxikologie und Umwelthygiene, TU Munchen, Lazarettstrasse 62, D-80636 Munchen, Germany
Recombinant V79 Chinese hamster cell lines have been constructed for the expression of useful functions, e.g. cytochromes P450, in order to study metabolism-dependent toxicity. Recombinant cell lines are derived as single clones upon gene transfer and selection from the parental V79 cell line. It is of fundamental importance for a reliable application of the recombinant cell lines to show that the original biological characteristics of the parental line are maintained. As part of these efforts, fluorescence in situ hybridization with Chinese hamster chromosome-specific DNA libraries was performed in order to identify the origin of the chromosomes from which a marker chromosome present in the recombinant cell line V79MZr2B1 was derived, which could not be identified by standard cytogenetic techniques.
4 To whom correspondence should be addressed. Tel: +39 50 577834; Fax +39 50 576661; Email: s.simi{at}imd.pi.cnr.it