Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Clare, G.
Right arrow Articles by Clements, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Clare, G.
Right arrow Articles by Clements, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Mutagenesis, Vol. 16, No. 2, 179-182, March 2001
© 2001 UK Environmental Mutagen Society/Oxford University Press

Industrial Genotoxicology Group (IGG): Cytotoxicity In Vitro,Royal Society of Medicine, London, UK, 6 December 1999

Gill Clare1 and Julie Clements2

1 AstraZeneca, Loughborough and 2 Covance Laboratories, Harrogate, UK


   Introduction
 
Some chemicals are not detectable in in vitro genotoxicity assays unless the concentrations tested are cytotoxic. However, excessive toxicity often does not allow a proper evaluation of the relevant genetic end point (CPMP/ICH, 1995Go). At very low survival levels in mammalian cells mechanisms other than direct genotoxicity per se can lead to `positive' results that are related to cytotoxicity and not genotoxicity. These considerations are conflicting. They have been balanced by defining a desired level of toxicity for the top concentrations of cytotoxic compounds in in vitro genotoxicity tests. However, recently the appropriate level of toxicity at the upper limit of testing in the in vitro chromosomal aberration test has been challenged (Galloway, 2000Go).

The objective of the meeting was to consider how cells die and measurement of cytotoxicity in relation to genotoxicity testing.


   Mechanisms
 
Kevin Chipman (University of Birmingham, Birmingham, UK) distinguished between necrosis and apoptosis and highlighted . . . [Full Text of this Article]


   Overview of how cytotoxicity is measured in each of the tests
 

   Practical experience
 

   IVCA results
 

   IVMNT results
 

   MLA results
 

   New/alternative ways of measuring cytotoxicity
 

   Summary
 

   References
 

Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?