Mutagenesis, Vol. 16, No. 6, 461-465,
November 2001
© 2001 UK Environmental Mutagen Society/Oxford University Press
Mutagenicity of a single 1-nitropyrene–DNA adduct N-(deoxyguanosin-8-yl)-1-aminopyrene in Escherichia coli located in a GGC sequence
Department of Chemistry, University of Connecticut, Storrs, CT 06269, USA
1-Nitropyrene, a common environmental pollutant, forms a major DNA adduct, N-(deoxyguanosin-8-yl)-1-amino- pyrene (dGAP). Mutational spectra of randomly introduced dGAP in Escherichia coli included different types of mutations, which depended on the base sequence surrounding the adduct. In earlier works we investigated the DNA sequence context effects of the adduct in repetitive CpG and non-repetitive CpGpC sequences. In the current work this adduct was incorporated into a non-repetitive GpGpC sequence in single-stranded M13mp7L2 DNA with the adduct located at either the 5' or 3' G. Potent genotoxicity of dGAP was evident from a significant reduction in the population of progeny phage following replication of these constructs in repair-competent E.coli cells. However, progeny derived from the 3'-GAP construct were much larger than those from the 5'-GAP construct. We suspect that a more facile translesion synthesis past the adduct at the 3' G relative to that at the 5' G, presumably due to a difference in conformation of dGAP in these two sites, might be responsible for this effect. With both adducted constructs, >95% of the progeny did not show any mutations at or near the adduct site, indicating highly efficient error-free translesion synthesis. However, a small population of mutants with one base deletions and base substitutions were detected. While the adduct induced –1 frameshifts (<1%) in each G site, base substitutions (1–2%), exhibiting predominantly G
C transversions, were detected only when the adduct was located at the 5' G. A comparison of the data from this study with a prior study in the CpGpC sequence suggests that dGAP mutagenesis is highly sensitive to the local DNA sequence and that a 5'-pyrimidine base might be important for targeted base substitutions by this adduct in E.coli.
1 To whom correspondence should be addressed: Tel: +1 860 486 3965; Fax: +1 860 486 2981; Email: ashis.basu{at}uconn.edu
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  P. Hilario, S. Yan, B. E. Hingerty, S. Broyde, and A. K. Basu Comparative Mutagenesis of the C8-Guanine Adducts of 1-Nitropyrene and 1,6- and 1,8-Dinitropyrene in a CpG Repeat Sequence. A SLIPPED FRAMESHIFT INTERMEDIATE MODEL FOR DINUCLEOTIDE DELETION J. Biol. Chem., November 15, 2002; 277(47): 45068 - 45074. [Abstract] [Full Text] [PDF]
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