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Mutagenesis, Vol. 17, No. 1, 73-77, January 2002
© 2002 UK Environmental Mutagen Society/Oxford University Press

Micronuclei and p53 accumulation in preneoplastic and malignant lesions of the head and neck

Valeria Delfino1, Gianluigi Casartelli1, Barbara Garzoglio, Marco Scala1, Paola Mereu1, Stefania Bonatti1,2,, Giovanni Margarino1 and Angelo Abbondandolo1,3

1 National Cancer Research Institute, Genova, Italy, 2 Institute of Mutagenesis and Differentiation of the CNR, Pisa, Italy and 3 Department of Oncology, Biology and Genetics, University of Genova, Genova, Italy

No single biomarker can predict the risk for malignant trasformation of precancerous lesions of the head and neck. Micronucleus frequency, nuclear p53 accumulation and mitotic index were determined in proliferating basal cells using paraffin-embedded specimens from normal, dysplastic and malignant tissues. p53 accumulation was detected by immunohistochemistry using pAb 1081 and pAb 240 antibodies. Micronuclei were scored in the same cell population and classified for the presence/ absence of p53 accumulation in the main nucleus. Fifty-three carcinomas and 15 precancerous lesions were studied. Both micronuclei and p53 accumulation were found in precancerous lesions, suggesting that they are early events in head and neck squamous cell carcinoma progression. The two biomarkers were not related to each other: indeed, micronucleus frequency was higher in p53-negative than in p53-positive cells. Three patients with precancerous lesions later developed carcinomas; all three cases showed high frequencies of both micronuclei and cells accumulating p53 protein.

4 To whom correspondence should be addressed at: National Cancer Research Institute, Largo R. Benzi 10, I-16132 Genova, Italy. Tel: +39 10 5600251; Fax: +39 10 5600992; Email: 'stefania bonatti'abbondan{at}hp380.ist.unige.it


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