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Mutagenesis, Vol. 17, No. 3, 189-191, May 2002
© 2002 UK Environmental Mutagen Society/Oxford University Press

Measurement of cyclobutane thymidine dimers in melanocytic nevi and surrounding epidermis in human skin in situ

Lonneke C. Wilms, Chunyan Zhao, Erna Snellman1, Dan Segerbäck and Kari Hemminki,2

Department of Biosciences at Novum, Karolinska Institute, 14157 Huddinge, Sweden and 1 Department of Dermatology, Päijät-Häme Central Hospital, FIN-15850 Lahti, Finland

The numbers of melanocytic nevi, localized benign proliferations of melanocytes, have been shown to be associated with an increased risk for development of melanoma. In the present study we have developed an alternative post-labelling method for determination of levels of cyclobutane thymidine dimers (T=T) as dinucleotides at sensitivities sufficient for analysis of human skin samples. Using the developed method, the induction of T=T was determined in melanocytic nevi in situ and surrounding skin, obtained from seven subjects, after exposure to solar simulating radiation. The T=T level in nevi was found to be 1- to 4.5-fold lower than that in surrounding skin and the difference was statistically significant (Student's t-test, P < 0.05).

2 To whom correspondence should be addressed. Tel: +46 8 6089243; Fax: +46 8 6081501; Email: kair hemminki{at}cnt.ki.se


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