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Mutagenesis, Vol. 17, No. 5, 399-403, September 2002
© 2002 UK Environmental Mutagen Society/Oxford University Press

SCE formation after exposure of CHO cells prelabelled with BrdU or biotin-dUTP to various DNA-damaging agents

Lubomir Stoilov, Andrzej Wojcik1,2, Ashok K. Giri3 and Günter Obe4,5

Institute of Genetics, Department of Molecular Genetics, 1113 Sofia, Bulgaria, 1 Institute of Nuclear Chemistry and Technology, 03-195 Warszawa, Poland, 2 Institute of Biology, Swietokrzyska Academy, 25-406 Kielce, Poland, 3 Indian Institute of Chemical Biology, 700 032 Calcutta, India and 4 Institute of Genetics, University of Essen, D-45117 Essen, Germany

Formation of sister chromatid exchanges (SCE) is a mechanism of repair or bypass of DNA damage during S phase. Although SCE have been studied for a long time, the types of DNA lesions involved and the role of 5-bromodeoxyuridine (BrdU) in SCE formation are a matter of debate. We have developed a novel method of differential labelling of sister chromatids with biotin-16–2'-deoxyuridine-5'-triphosphate (biotin-dUTP) and could show that a substantial proportion of radiation-induced SCE arise via damage to BrdU-moieties. The present investigations were performed to examine the role of BrdU in the formation of SCE by the endonucleases AluI and DNase I, as well as the alkylating agent mitomycin C (MMC). CHO cells unifilarily prelabelled with biotin-dUTP or BrdU were treated and the frequencies of SCE analysed in the first post-treatment mitoses. AluI induced similar frequencies of SCE in cells prelabelled with BrdU or biotin-dUTP. DNase I induced significantly more SCE in cells prelabelled with BrdU than with biotin-dUTP. MMC induced slightly more SCE in cells labelled with biotin-dUTP than BrdU, but the difference was not significant. The possible mechanisms responsible for the enhanced SCE frequency following DNase I treatment of cells prelabelled with BrdU are discussed.

5 To whom correspondence should be addressed. Tel: 49 201 183 3388; Fax: 49 201 183; Email: guenter.obe{at}uni-essen.de


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