Mutagenesis vol. 18 no. 4 pp. 371-376,
July 2003
© 2003 UK Environmental Mutagen Society/Oxford University Press
Nutritional supplementation with antioxidants decreases chromosomal damage in humans
inská2
imírová
oková
olováInstitute of Preventive and Clinical Medicine, Limbova 14, 83301 Bratislava, Slovak Republic and 1Institute for Nutrition Research, University of Oslo, POB1046 Blindern, N-0316 Oslo, Norway
In order to investigate the effects of antioxidant supplementation on chromosome damage, a 3 month antioxidant supplementation trial was conducted on groups of 28 myocardial infarction survivors and 57 rural controls, all male. The supplement consisted of vitamin C (100 mg/day), vitamin E (100 mg/day), ß-carotene (6 mg/day) and selenium (50 µmg/day). Dietary antioxidants in plasma were measured, as well as the ferric reducing ability of plasma (a measure of total plasma antioxidant status) and the concentration of malondialdehyde as an indicator of oxidative stress. Lymphocytes collected at the beginning and end of the supplementation period were stimulated to proliferate and metaphases accumulated for scoring of chromosome aberrations: per cent aberrant cells and chromatid and chromosome breaks. Supplementation with antioxidants was associated with a decrease in the percentage of cells with chromosome aberrations in the group of rural controls (0.63% before compared with 0.27% after supplementation; P = 0.03). The largest effect of supplementation was seen in smokers in this group (0.12% aberrant cells in supplemented compared with 0.81% in placebo group; P > 0.001). The results support the hypothesis that antioxidants decrease genetic damage.
2To whom correspondence should be addressed. Email: dusinska{at}upkm.sk
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