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Mutagenesis vol. 19 no. 4 pp. 285-290, July 2004
© 2004 UK Environmental Mutagen Society/Oxford University Press

The use of EBV-transformed cell lines of breast cancer patients to measure chromosomal radiosensitivity

A. Baeyens1, H. Thierens1, K. Vandenbulcke2, L. De Ridder1 and A. Vral1

1Department of Anatomy, Embryology, Histology and Medical Physics, University of Gent, L. Pasteurlaan 2, B-9000 Gent, Belgium and 2Department of Radiopharmacy, University of Gent, Harelbekestraat 72, B-9000 Gent, Belgium

To investigate the chromosomal radiosensitivity of lymphocytes in cancer patients the micronucleus (MN) assay is often used and performed on freshly drawn peripheral blood lymphocytes. The use of Epstein–Barr virus (EBV)-transformed lymphoblastoid cell lines may have a lot of advantages (e.g. large pool of cells) compared with fresh blood samples. In this study we have investigated whether the response of EBV-transformed lymphoblastoid cell lines to irradiation in the G1/S/G2 phases of the cell cycle is the same as in concordant whole blood cultures where primary lymphocytes were irradiated in the G0 phase of the cell cycle. For this study the MN assay (2 Gy) was performed on EBV-transformed cell lines of breast cancer patients and a group of healthy women. Those breast cancer patients were selected who showed an elevated chromosomal radiosensitivity in fresh blood samples in a previous study. The results demonstrated that the enhanced chromosomal radiosensitivity observed in fresh blood cultures of breast cancer patients is not present in EBV-transformed cell lines derived from the same blood samples. Therefore, care must be taken when EBV cell lines are used to assess chromosomal radiosensitivity in breast cancer patients.

3To whom correspondence should be addressed. Tel: +32 9 264 92 48; Fax: +32 9 264 94 98; Email: anne.vral{at}UGent.be

Received on September 8, 2003; revised on March 4, 2004; accepted on March 18, 2004


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