Skip Navigation


Mutagenesis Advance Access originally published online on June 14, 2005
Mutagenesis 2005 20(4):279-283; doi:10.1093/mutage/gei034
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
20/4/279    most recent
gei034v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (5)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Franke, S. I. R.
Right arrow Articles by da Silva, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Franke, S. I. R.
Right arrow Articles by da Silva, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?


© The Author 2005. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. All rights reserved. For permissions, please email: journals.permissions@oupjournals.org

Influence of orange juice over the genotoxicity induced by alkylating agents: an in vivo analysis

Silvia Isabel Rech Franke1,2, Daniel Prá2, Bernardo Erdtmann2,3, João Antonio Pêgas Henriques2,3,4 and Juliana da Silva2,4,*

1Curso de Nutrição, Departamento de Educação Física e Saúde, Universidade de Santa Cruz do Sul (UNISC), Santa Cruz do Sul, RS, Brazil, 2Genotox/Centro de Biotecnologia/Programa de Pós-Graduação em Biologia Celular e Molecular/Programa de Pós-Graduação em Genética e Biologia Molecular, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, R S, Brazil, 3Instituto de Biotecnologia, Universidade de Caxias do Sul (UCS), Caxias do Sul, RS, Brazil and 4Curso de Biologia/Curso de Farmácia, Universidade Luterana do Brasil (ULBRA), Canoas, RS, Brazil

There is considerable epidemiological evidence indicating an association between diets rich in fresh fruit and vegetables and a decreased incidence of cancers. Methyl methanesulfonate (MMS) and cyclophosphamide (CP) are alkylating agents that differ in their mode of action. MMS is a directly-acting, monofunctional agent, while CP is a bifunctional agent that requires metabolic activation to a reactive metabolite. To evaluate if orange juice could reduce DNA damage induced by these alkylating agents, mice were treated orally (by gavage) with MMS and CP, prior to and after treatment with orange juice. DNA damage was evaluated by the comet assay in peripheral white blood cells. Under these experimental conditions, orange juice reduced the extent of DNA damage caused by both mutagens. For MMS, the antigenotoxic effect of the orange juice was both protective (orange juice pre-treatment) and reparative (orange juice post-treatment); for CP, the effect was reparative only. The components of orange juice can have several biological effects, including acting as targets of toxicants and modulating metabolization/detoxification routes. Considering the different mechanisms of the action of the two drugs, different protective effects are suggested. These results demonstated the ability of the in vivo comet assay to detect in vivo modulation of MMS and CP mutagenicity by orange juice.

* To whom correspondence should be addressed. Laboratório de Genética Toxicológica, Departamento de Biologia- PPGECIM, Universidade Luterana do Brasil (ULBRA); R. Miguel tostes, 101, Prédio 14, Sala 230; CEP 92420-280, Canoas, RS, Brasil. Tel/Fax: +55 51 3387 1138; Email: juliana.silva{at}ulbra.br


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
S.-Y. Kim, S.-G. Kim, Y.-S. Kim, P. J. Seo, M. Bae, H.-K. Yoon, and C.-M. Park
Exploring membrane-associated NAC transcription factors in Arabidopsis: implications for membrane biology in genome regulation
Nucleic Acids Res., January 12, 2007; 35(1): 203 - 213.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.