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Mutagenesis Advance Access originally published online on January 17, 2007
Mutagenesis 2007 22(2):117-122; doi:10.1093/mutage/gel061
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© The Author 2007. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Adaptive response to DNA and chromosomal damage induced by X-rays in human blood lymphocytes

LM Stoilov*, LHF Mullenders1, F Darroudi1 and AT Natarajan1,2

Department of Molecular Genetics, Institute of Genetics "Acad. D. Kostoff" BAS, Sofia 1113, Bulgaria 1Department of Toxicogenetics, Leiden University Medical Centre, Leiden, The Netherlands 2Department of Agrobiology and Agrochemistry, University of Tuscia, Viterbo, Italy

Nucleoid sedimentation, single-cell gel electrophoresis (comet assay) and premature chromosome condensation (PCC) technique were utilized to estimate the involvement of DNA strand breaks and chromosomal damage in radio-adaptive response of stimulated human lymphocytes. Conditioning of cells with 0.02 Gy X-rays rendered them more resistant to single- and double-strand DNA breaks produced by 1 Gy challenging treatment as revealed by the sedimentation behaviour of the nucleoids and the comet assay. Nucleoid sedimentation also demonstrated that adaptive reaction towards X-ray-induced DNA damage is favoured in the presence of oxygen. A concomitant decrease in the amount of interphase chromosomal breaks visualized by PCC under the same experimental conditions was observed. Data indicate that adaptation of human lymphocytes to X-rays is tightly linked to the reduced susceptibility towards generation of DNA and chromosomal breaks. It is proposed that the very persistence of DNA strand discontinuities might serve as a triggering signal for the adaptation of human lymphocytes against ionizing radiation exposure.

* To whom correspondence should be addressed. Tel: +359(2)9746228(348); Fax: +359(2)9785516; Email: molgen{at}bas.bg

Received on September 12, 2006; revised on October 26, 2006; accepted on October 31, 2006.


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