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Mutagenesis Advance Access originally published online on November 13, 2007
Mutagenesis 2008 23(1):19-26; doi:10.1093/mutage/gem028
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© The Author 2007. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org.

Fast repair of oxidative DNA damage by phenylpropanoid glycosides and their analogues

Yimin Shi1, Wengfeng Wang3, Chungyang Huang1, Zhongjian Jia2, Side Yao3 and Rongliang Zheng1,4,*

1School of Life Sciences 2College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P.R. China 3Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P.R. China 4E-Institutes of Shanghai Municipal Education Commission, Shanghai 200000, P.R. China

The repair activities and reaction mechanisms of phenylpropanoid glycosides (PPGs) and their analogues, isolated from Chinese folk medicinal herbs, towards oxidative DNA damage were studied with pulse radiolytic technique. On pulse irradiation of nitrogen-saturated 4 mM poly C aqueous solution containing one of the tested polyphenols, 40 mM K2S2O8 and 200 mM t-BuOH, the transient absorption spectrum of the oxidative radical of poly C decays with the concurrent formation of the phenoxyl radical of the tested polyphenols within several tens of microseconds after the electron pulse irradiation. The result indicated that there was a repair reaction between oxidative radical of poly C and the tested polyphenols. The repair activities also were observed for the tested polyphenols towards the radical cations of single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). The rate constants were determined to be 3.7–6.4 x 109, 4.8–5.5 x 108 and 8.8–10.3 x 108 M–1·sec–1 for the repair reactions of oxidative radical of poly C and radical cations of ssDNA and dsDNA, respectively. The result of this study together with those of our previous studies demonstrates that PPGs and their analogues can fast repair not only the damage of deoxynucleoside and deoxynucleotide but also the damage of integral DNA, with the latter being closer to a cellular condition.

* To whom correspondence should be addressed. Tel: +86 0931 8912563; Email: zhengrl{at}lzu.edu.cn

Received on April 4, 2007; revised on June 22, 2007; accepted on June 24, 2007.


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