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Mutagenesis Advance Access published online on March 8, 2005
Mutagenesis, doi:10.1093/mutage/gei016
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Published by Oxford University Press on behalf of the UK Environmental Mutagen Society 2005.
Received November 22, 2004
Revised February 4, 2005
Accepted February 4, 2005

Original article

Resistance to the antibiotic Zeocin by stable expression of the Sh ble gene does not fully suppress Zeocin-induced DNA cleavage in human cells

Manel Oliva Trastoy 1, Martine Defais 1, and Florence Larminat 1*

1 IPBS, UMR 5089 CNRS, 205 Route de Narbonne, 31077 Toulouse, France

* To whom correspondence should be addressed.
Florence Larminat, E-mail: Florence.Larminat{at}cict.fr


  Abstract

Zeocin is a member of the bleomycin/phleomycin family of antibiotics, known to bind and cleave DNA. We established human SK-OV-3 cells that stably express the Zeocin resistance gene (Sh ble) using an ecdysone-inducible mammalian expression system. Surprisingly, our results demonstrated that Zeocin, added in the culture medium to maintain the expression of the ecdysone receptor, was responsible for the formation of DNA strand breaks in the recombinant cells. This suggests that the Zeocin is not completely detoxified and is still able to cleave DNA, despite the stable expression of the Sh ble gene in the recombinant clones. Our study indicates that one needs to be very cautious in the interpretation of data involving stable cell lines selected with Zeocin.


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