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Mutagenesis Advance Access published online on November 23, 2005
Mutagenesis, doi:10.1093/mutage/gei065
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© The Author 2005. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org
Received September 8, 2005
Revised October 25, 2005
Accepted October 26, 2005

Original article

The protective effect of L-carnitine in peripheral blood human lymphocytes exposed to oxidative agents

Claudia Lorenti Garcia 1, Silvia Filippi 1, Pasquale Mosesso 1, Menotti Calvani 2, Raffaella Nicolai 2, Luigi Mosconi 2, and Fabrizio Palitti 1 *

1 Laboratory of Molecular Cytogenetic and Mutagenesis, DABAC, Università degli Studi della Tuscia, Via S.Camillo de Lellis, I-01100 Viterbo, Italy
2 Direzione scientifica SIGMA-TAU, Via Pontina Km. 30, 400, I-00040 Pomezia, Rome, Italy

* To whom correspondence should be addressed.
Fabrizio Palitti, E-mail: palitti{at}unitus.it


  Abstract

Literature data indicate L-carnitine (LC), a trans-mitochondrial carrier of acetyl and long chain groups, as an agent possessing protective effects against oxidative stress in mammalian cells. However, the major factor involved in the protective mechanism is not known. The protection activity exerted by this agent against reactive oxygen species induced by hydrogen peroxide (H2O2) and t-butylhydroperoxide (t-butyl-OOH) treatment in isolated human peripheral blood lymphocytes (PBLs) has been studied. Human lymphocytes cells were isolated and pre-incubated with 5 mM LC before H2O2 (100 µM) and t-butyl-OOH (400 µM) treatment. The protective effect of LC on treated PBLs was measured by single cell gel electrophoresis and the analysis of chromosomal aberrations. Results show that LC treated cells exhibited a significant decrease in the number of oxidative induced single-strand breaks and chromosomal aberrations.


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