Mutagenesis Advance Access published online on April 13, 2006
Mutagenesis, doi:10.1093/mutage/gel016
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1 Department of Experimental and Applied Medicine, Institute of Preventive and Clinical Medicine, Research Base of Slovak Medical University, Limbová 14, 83303 Bratislava, Slovakia
* To whom correspondence should be addressed. Participants in a study of occupational exposure to mineral fibres in Slovakia were analysed for the polymorphism 23A
Received December 20, 2005
Revised February 20, 2006
Accepted March 2, 2006
Original article
Possible involvement of XPA in repair of oxidative DNA damage deduced from analysis of damage, repair and genotype in a human population study
Mária Du
inská 1 *,
Zuzana D
upinková 1,
Ladislava Wsólová 1,
Vikki Harrington 2,
and
Andrew R. Collins 3
2 School of Life Sciences, Robert Gordon University, Aberdeen, UK
3 School of Life Sciences, Robert Gordon University, Aberdeen, UK; Department of Nutrition, University of Oslo, Oslo, Norway
Mária Duinská, E-mail: maria.dusinska{at}szu.sk
Abstract 
G in the DNA repair gene XPA. Of the 388 subjects, 239 were exposed to asbestos, stonewool or glass fibre; the rest were unexposed controls. Levels of DNA base alterations (oxidation and alkylation) in lymphocytes were measured using the comet assay with lesion-specific endonucleases. 8-oxoguanine DNA glycosylase (OGG1) DNA repair activity was measured, as incision activity of a lymphocyte extract on DNA containing the OGG1 substrate 8-oxoguanine. Presence of the A allele was associated with higher levels of DNA damage (sites sensitive to formamidopyrimidine DNA glycosylase, endonuclease III or 3-methyladenine DNA glycosylase II) as well as with higher activity of OGG1 repair enzyme. DNA base damage increased with age, showing highly significant correlations when the whole population or subgroups of the population were analysed. OGG1 repair activity also increased with age, but when analysed according to XPA genotype, the increase was observed only in those individuals with an A allele. Although XPA is known as a protein involved in nucleotide excision repair of UV-induced damage and bulky DNA adducts, it may also have a role in the repair of oxidized bases.
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