Mutagenesis Advance Access published online on May 30, 2006
Mutagenesis, doi:10.1093/mutage/gel023
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department of Radiobiology, Life Sciences Center, Kasturba Medical College, Manipal 576 104, Karnataka, India
* To whom correspondence should be addressed. Coleus aromaticus (Benth, Family: Laminaceae), Indian Oregano native to India and Mediterranean, is well known for its medicinal properties. A preliminary study was undertaken to elucidate in vitro free radical scavenging potential and inhibition of lipid peroxidation by C.aromaticus hydroalcoholic extract (CAE). Anti-clastogenic and radioprotective potential of CAE were studied using micronucleus assay after irradiating Chinese hamster fibroblast (V79) cells. CAE at 10, 20, 40, 60, 80, 100 and 120 µg/ml resulted in a dose-dependent increase in radical scavenging ability against various free radicals viz., 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), superoxide anion (O2•-), hydroxyl (OH•) and nitric oxide (NO•) generated in vitro. A maximum scavenging potential was noticed at 100 µg/ml and a saturation point was reached thereafter with the increasing doses of CAE. The free radical scavenging potential of the extract was in the order of DPPH > ABTS > Superoxide > Hydroxyl > Nitric oxide. CAE also exhibited a moderate inhibition of lipid peroxidation in vitro, with a maximum inhibition at 60 µg/ml (33%), attaining saturation at higher doses. The extract also rendered protection against radiation induced DNA damage, as evidenced by the significant (P < 0.05) decrease in the percentage of radiation-induced micronucleated cells (MN) and frequency of micronuclei (total). A maximum anticlastogneic effect/ radioprotection was noticed at a very low concentration i.e., 5 µg/ml of CAE, treated 1 h prior to 2 Gy of gamma radiation. A significant (P < 0.0001) anticlastogenic/radioprotective effect was also observed when the cells were treated with an optimum dose of CAE (5 µg/ml) 1 h prior to 0.5, 1, 2 and 4 Gy of gamma radiation compared with the respective radiation control groups. Overall, our results established an efficient antioxidant, anticlastogenic and radioprotective potential of CAE, which may be of great pharmacological importance.
Received February 20, 2006
Revised March 24, 2006
Accepted April 12, 2006
Original article
Antioxidant, anticlastogenic and radioprotective effect of Coleus aromaticus on Chinese hamster fibroblast cells (V79) exposed to gamma radiation
B. S. Satish Rao 1 *,
R. Shanbhoge 1,
D. Upadhya 1,
G. C. Jagetia 1,
S. K. Adiga 2,
P. Kumar 2,
K. Guruprasad 2,
and
P. Gayathri 1
2 Department of Obstetrics and Gynecology, Kasturba Medical College, Manipal 576 104, Karnataka, India
B. S. Satish Rao, E-mail: raokmc{at}yahoo.com
Abstract 
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  B. Rao, R Shanbhoge, B. Rao, S. Adiga, D Upadhya, B. Aithal, and M. Kumar Preventive efficacy of hydroalcoholic extract of Cymbopogon citratus against radiation-induced DNA damage on V79 cells and free radical scavenging ability against radicals generated in vitro Human and Experimental Toxicology, April 1, 2009; 28(4): 195 - 202. [Abstract] [PDF]
|
|