Skip Navigation



Mutagenesis Advance Access published online on December 8, 2006
Mutagenesis, doi:10.1093/mutage/gel053
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
22/1/69    most recent
gel053v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Schmid, O.
Right arrow Articles by Speit, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schmid, O.
Right arrow Articles by Speit, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org
Received September 13, 2006
Revised October 5, 2006
Accepted October 14, 2006

Original article

Genotoxic effects induced by formaldehyde in human blood and implications for the interpretation of biomonitoring studies

Oliver Schmid 1 and Günter Speit 1 *

1 Institut für Humangenetik, Universität Ulm, D-89069 Ulm, Germany

* To whom correspondence should be addressed.
Günter Speit, E-mail: guenter.speit{at}uni-ulm.de


  Abstract

Formaldehyde (FA) was tested for its genotoxicity in human blood cultures. We treated blood samples at the start of the culture to follow FA-induced DNA damage (DNA-protein crosslinks, DPX), its repair and its genetic consequences in form of sister chromatid exchanges (SCE) and micronuclei (MN). Our results clearly indicate that DPX (determined by the comet assay) are induced at FA concentrations of ≥25 µM. DPX induced by FA concentrations up to 100 µM are completely removed before lymphocytes start to replicate. SCE are induced at concentrations >100 µM parallel to the induction of cytotoxicity (measured as reduction of the replication index). MN were not induced by FA concentrations up to 250 µM (the highest analyzable concentration) added at the start of the blood cultures in the cytokinesis-block micronucleus (CBMN) test. FA-induced cytotoxicity (measured as reduction of the nuclear division index) possibly prevented division of damaged cells. MN were only significantly induced in human blood when proliferating cells were exposed to FA during the last cell cycle before preparation. Several human biomonitoring studies reported increased frequencies of SCE and MN in lymphocytes of subjects exposed to FA. Our results characterize the genotoxic potential of FA in cultured lymphocytes and lead to the conclusion that cytogenetic effects of FA are very unlikely to occur in blood cultures of FA-exposed subjects.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 MutagenesisHome page
S. Neuss and G. Speit
Further characterization of the genotoxicity of formaldehyde in vitro by the sister chromatid exchange test and co-cultivation experiments
Mutagenesis, May 13, 2008; (2008) gen025v1.
[Abstract] [Full Text] [PDF]

Home page
 MutagenesisHome page
G. Speit, P. Schutz, J. Hogel, and O. Schmid
Characterization of the genotoxic potential of formaldehyde in V79 cells
Mutagenesis, November 1, 2007; 22(6): 387 - 394.
[Abstract] [Full Text] [PDF]

Home page
 MutagenesisHome page
W. Wang, J. Xu, L. Xu, B. Yue, and F. Zou
The instability of (GpT)n and (ApC)n microsatellites induced by formaldehyde in Escherichia coli
Mutagenesis, September 1, 2007; 22(5): 353 - 357.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.