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Mutagenesis Advance Access published online on June 11, 2008
Mutagenesis, doi:10.1093/mutage/gen031
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© The Author 2008. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org.

Evaluation of genetic damage in a Brazilian population occupationally exposed to pesticides and its correlation with polymorphisms in metabolizing genes

Juliana da Silva1, Camila R. Moraes2, Vanina D. Heuser3,4, Vanessa M. Andrade3,5, Fernanda R. Silva3, Kátia Kvitko3, Vanessa Emmel3, Paula Rohr3, Diana Lilian Bordin6, Ana Cristina Andreazza6, Mirian Salvador6, João A. P. Henriques1,2,3,6,* and Bernardo Erdtmann6

1Laboratório de Genética Toxicológica, PPGGTA e PPGECIM, Universidade Luterana do Brasil, Canoas-RS, Brazil 2Departamento de Biofísica, Programa de Pós-Graduação em Biologia Celular e Molecular 3Departamento de Genética, Programa de Pós-Graduação em Genética e Biologia Molecular, Universidade Federal do Rio Grande do Sul, Porto Alegre-RS, Brazil 4Division of Biochemical Toxicology, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden 5Laboratório de Imunologia e Mutagênese, Programa de Pós-Graduação em Ciências da Saúde, Universidade do Extremo Sul Catarinense, Criciúma-SC, Brazil 6Instituto de Biotecnologia, Universidade de Caxias do Sul, Caxias do Sul, RS, Brazil

Cytogenetic damage in individuals occupationally exposed to pesticides has received the attention of investigators in several countries, but no definitive conclusions can yet be made. The present study aimed at assessing if prolonged exposure to complex mixtures of pesticides leads to an increase in cytogenetic damage. Vineyard workers exposed to pesticides in Caxias do Sul (Brazil) were evaluated using the micronucleus (MN) test in binucleated lymphocytes and the comet assay in peripheral leukocytes. In order to evaluate if genetically determined individual variations in xenobiotic metabolizing capacity could modify individual susceptibility to the possible genotoxic effects of pesticides, the subjects were genotyped for several genes: GSTT1, GSTM1, GSTP1, CYP1A1, CYP2E1 and PON. The study involved a total number of 173 men: 108 were agricultural workers exposed to pesticides and 65 were controls. The present study showed a high rate of MN and DNA damage in pesticide-exposed individuals (P ≤ 0.001; Mann–Whitney U-test). In addition, some effects of genetic polymorphisms in PON in the modulation of MN results were observed in the exposed group, and an association between GSTM1, GSTT1 and CYP2E1 polymorphisms was suggested.

* To whom correspondence should be addressed. Tel: +55 51 33166069; Fax: +55 51 33166084; Email: pegas{at}cbiot.ufrgs.br

Received on September 4, 2007; revised on April 24, 2008; accepted on April 25, 2008.


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