Mutagenesis Advance Access published online on May 15, 2009
Mutagenesis, doi:10.1093/mutage/gep017
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Comparison of the Ames II and traditional Ames test responses with respect to mutagenicity, strain specificities, need for metabolism and correlation with rodent carcinogenicity
Xenometrix AG, Gewerbestrasse 25, CH-4123 Allschwil, Switzerland 1Experimental Toxicology and Ecology, BASF SE Product Safety 2Regulations, Toxicology and Ecology, BASF SE Product Safety, 67056 Ludwigshafen am Rhein, Germany 3Errol Zeiger Consulting, Chapel Hill, NC 27514, USA
The Ames II Salmonella mutagenicity assay procedure was used to test 71 chemicals, and the results were compared with those from the traditional Ames Salmonella test using the NTP database as the reference. All Ames II tests were performed using a fluctuation procedure in microplate format, using TAMix for the detection of base pair substitutions and TA98 to detect frameshift mutations. There was 84% agreement between the two procedures in identifying mutagens and non-mutagens, which is equivalent to the intra- and interlaboratory reproducibility of 87% for the traditional test. The two tests also performed similarly in their predictions of rodent carcinogenicity.
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Received on March 17, 2009; revised on April 14, 2009; accepted on April 20, 2009.